Biochem/physiol Actions
Hydrolyzes α(2→3), α(2→6), and α(2→8)-glycosidic linkages of terminal sialic residues of various glycomolecules.
Releases α(2→3)- and α(2→6)-linked N-acetylneuraminic acid from complex oligosaccharides.
Packaging
Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).
Preparation Note
Expressed in glycosidase-free hosts.
Unit Definition
One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C
One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin unless otherwise specified.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, and 25 mM NaCl.
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