N6514
Sigma

Neuraminidase from Vibrio cholerae

Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

Synonym

Acyl-neuraminyl Hydrolase

Receptor-destroying enzyme

Sialidase

CAS Number

9001-67-6

Enzyme Commission (EC) Number

3.2.1.18 ( BRENDA | IUBMB )

MDL number

MFCD00131711

EG/EC Number

232-624-6

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Descriptions

Application

Neurminidase is used as a cell-surface probe for glycoconjugate distribution¹ and in substrate specificity studies.²

Preparation Note

A further purification by affinity chromatography of our Type III.

Quality

Preservative free.

Unit Definition

One unit will liberate 1.0μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride.

Properties

sterility

sterile-filtered

type

Type II

form

buffered aqueous solution

foreign activity

Protease and NAN-aldolase, present

storage temp.

2-8°C

References

Cited Reference

1. Whiteheart, S.W., and Hart, G.W. Anal. Biochem. 163, 123, (1987)

2. Shukla, A.K., and Schauer, R. Anal. Biochem. 158, 158, (1986)

Reference

Cassidy, J.T., et al. J. Biol. Chem. 240, 3501, (1965)

Safety

WGK Germany

RTECS

QQ3450000