N6786
Sigma

Monoclonal Anti-Notch1 antibody produced in mouse

clone mN1A, purified immunoglobulin, buffered aqueous solution

MDL number

MFCD03455659

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Descriptions

Immunogen

recombinant protein containing the cdc10-NCR region of mouse Notch1.

Specificity

Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

mN1A

form

buffered aqueous solution

species reactivity

mouse, human

application(s)

immunoblotting: 1-2 μg/mL using extracts of cells stably transfected with a truncated Notch1 (32D-mN1-ICDOP that expresses the intracellular mouse Notch1 molecule-65 kDa).

immunocytochemistry: suitable

indirect ELISA: suitable

microarray: suitable

isotype

IgG1

Gene Information

mouse ... Notch1(18128)

human ... NOTCH1(4851)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Milner, L.A., et al. Proc. Natl. Acad. Sci. USA 93, 13014-13019, (1996) abstract

Huppert, S.S., et al. Nature 405, 966-970, (2000) abstract

Safety

WGK Germany

NWG

Related product

N4788, Anti-NOTCH1 antibody produced in rabbit

Array

XP725, Panorama^® Antibody Microarray - XPRESS Profiler725 Kit

Related Categories

... Functional Profiling of Stem Cells > Antibodies against Proteins/Bioactives/Markers/Receptors

... Stem Cell Characterization > Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology

... Stem Cell Differentiation > Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology

... Antibodies for Neurobiology > Antibodies to Neural Proteins

... Neuronal Development > Antibodies to Notch Signaling

... Clone Index > M

... Alphabetical Index > N

... Neural Stem Cell Tracking > Neural Markers

N6786 - Datasheet (26kb)

MSDS

Certificate of Analysis

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