N7265
Sigma

Nitrate Reductase (NAD[P]H) from Aspergillus niger

lyophilized powder, ≥300 units/g solid

Synonym

NAD(P)H:Nitrate oxidoreductase

CAS Number

9029-27-0

Enzyme Commission (EC) Number

1.7.1.2 ( BRENDA | IUBMB )

MDL number

MFCD00131725

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Descriptions

Biochem/physiol Actions

Catalyzes the reduction of NAD and NADP to NADH and NADPH, respectively.

Unit Definition

One unit will reduce 1.0 μmole of nitrate per min in the presence of β-NADPH at pH 7.5 at 25 °C.

Properties

form

lyophilized powder

storage temp.

2-8°C

References

Reference

Chiang, R.C., et al., ‘Locked-on’ and ‘locked-off’ signal transduction mutations in the periplasmic domain of the Escherichia coli NarQ and NarX sensors affect nitrate- and nitrite-dependent regulation by NarL and NarP. Mol. Microbiol. 24, 1049-1060, (1997) abstract

Unden, G., and Schirawski, J., The oxygen-responsive transcriptional regulator FNR of Escherichia coli: the search for signals and reactions. Mol. Microbiol. 25, 205-210, (1997) abstract

Meyer, C., et al., Mutational and structural analysis of the nitrate reductase heme domain of Nicotiana plumbaginifolia. J. Biol. Chem. 266, 20561-20566, (1991) abstract

Safety

WGK Germany