N7271
Sigma

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

Synonym

Acyl-neuraminyl Hydrolase

Acylneuraminyl hydrolase

Receptor-destroying enzyme

Sialidase

CAS Number

9001-67-6

Enzyme Commission (EC) Number

3.2.1.18 ( BRENDA | IUBMB )

MDL number

MFCD01092203

EG/EC Number

232-624-6

Expand/Collapse All

Descriptions

Biochem/physiol Actions

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Preparation Note

Expressed in glycosidase-free hosts.

Unit Definition

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl.

Properties

form

buffered aqueous solution

activity

≥135 units/mg protein

foreign activity

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

Gene Information

Streptococcus pneumoniae R6 ... nanA(933902), nanB(933504)

storage temp.

2-8°C

References

Reference

Cassidy, J.T., et al. J. Biol. Chem. 240, 3501, (1965)

Safety

WGK Germany

RTECS

QQ3450000

Related Categories

... Carbohydrate hydrolysis & PTM analysis > Carbohydrate and PTM Analysis

... Carbohydrate hydrolysis & PTM analysis > Carbohydrate Hydrolysis

... Carbohydrate-active Enzymes > Exoglycosidases

... 3.x.x.x Hydrolases > 3.2.x.x Glycosidases

N7271 - Datasheet (60kb)

MSDS

Certificate of Analysis

Certificate of Origin

More Information

Related Categories

Printer Friendly View

Ask A Scientist

Email Page

Last 5 Products Viewed

N7271 (Sigma)