N7885
Sigma

Neuraminidase from Vibrio cholerae

Type III, buffered aqueous solution, sterile-filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

Synonym

Acyl-neuraminyl Hydrolase

Receptor-destroying enzyme

Sialidase

CAS Number

9001-67-6

Enzyme Commission (EC) Number

3.2.1.18 ( BRENDA | IUBMB )

MDL number

MFCD00131711

EG/EC Number

232-624-6

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Descriptions

Application

Neurminidase is used as a cell-surface probe for glycoconjugate distribution¹ and in substrate specificity studies.²

Preparation Note

Chromatographically purified.

Quality

Preservative free.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl₂.

Properties

sterility

sterile-filtered

type

Type III

form

buffered aqueous solution

foreign activity

Protease and NAN-aldolase, present

storage temp.

2-8°C

References

Cited Reference

1. Whiteheart, S.W., and Hart, G.W. Anal. Biochem. 163, 123, (1987)

2. Shukla, A.K., and Schauer, R. Anal. Biochem. 158, 158, (1986)

Reference

Cassidy, J.T., et al. J. Biol. Chem. 240, 3501, (1965)

Safety

WGK Germany

RTECS

QQ3450000