Nitrocellulose membranes for blotting 

Nitrocellulose membranes are the most popular membranes for immunoblotting (Western blotting) of proteins. The basis of protein binding to nitrocellulose is believed to be due to hydrophobic interactions. The 0.45 μm pore size is the most commonly used for proteins greater than 10,000 MW, but for smaller proteins 0.2 μm is recommended to more efficiently trap the proteins.¹

15 cm × 15 cm

0.45 μm

1. Lin, J.C. and Kasamatsu, H., On the electrotransfer of polypeptides from gels to nitrocellulose membranes. Anal. Biochem. 128, 302-311, (1983)

Van Oss, C.J., et al., Mechanism of DNA (Southern) and protein (Western) blotting on cellulose nitrate and other membranes. J. Chromatogr. 391, 53-65, (1987)

Gershoni, J.M. and Palade, G.E., Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. Anal. Biochem. 124, 396-405, (1982)

UN 3270 4.1/PG 2

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