N8271
Sigma

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

recombinant, expressed in Escherichia coli, buffered aqueous solution

Synonym

Acyl-neuraminyl Hydrolase

Neuraminidase from Arthrobacter ureafaciens

Sialidase

CAS Number

9001-67-6

Enzyme Commission (EC) Number

3.2.1.18 ( BRENDA | IUBMB )

MDL number

MFCD00131711

EG/EC Number

232-624-6

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Descriptions

Biochem/physiol Actions

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Preparation Note

Expressed in glycosidase-free hosts.

Unit Definition

One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, and 25 mM NaCl.

Properties

recombinant

expressed in Escherichia coli

form

buffered aqueous solution

activity

≥135 units/mg protein

foreign activity

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

storage temp.

2-8°C

Safety

WGK Germany

RTECS

QQ3450000

3-10

Related Categories

... Carbohydrate hydrolysis & PTM analysis > Carbohydrate and PTM Analysis

... Carbohydrate hydrolysis & PTM analysis > Carbohydrate Hydrolysis

... Carbohydrate-active Enzymes > Exoglycosidases

... 3.x.x.x Hydrolases > 3.2.x.x Glycosidases

N8271 - Datasheet (104kb)

MSDS

Certificate of Analysis

Certificate of Origin

More Information