N8630
Sigma

Nuclease P₁ from Penicillium citrinum

lyophilized powder, ≥200 units/mg protein (E1%/280, 3'-5'-Phosphodiesterase)

Synonym

Endonuclease P₁

CAS Number

54576-84-0

Enzyme Commission (EC) Number

3.1.30.1 ( BRENDA | IUBMB )

MDL number

MFCD00131010

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Descriptions

Unit Definition

3'-5'-Phosphodiesterase: One unit will liberate 1.0 μmole of acid soluble nucleotides from RNA per min at pH 5.3 at 37 °C.
3'-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3′-AMP per min at pH 7.2 at 37 °C.

Properties

form

lyophilized powder

secondary activity

≥1,000 units/mg protein 3′-nucleotidase

packaging

vial of ≥250 units (using RNA substrate)

storage temp.

2-8°C

Safety

Safety Statements

22-24/25

WGK Germany

10-21

Related Categories

... General Metabolic Enzymes > Nucleotide Metabolism

... Application Index > Protein & Nucleic Acid Isolation

... 3.x.x.x Hydrolases > 3.1.x.x Acting on esters

N8630 - Product Information Sheet (26kb)

MSDS

Specification Sheet

Certificate of Analysis

Certificate of Origin

More Information

Nuclease P1 from Penicillium citrinum

lyophilized powder, ≥200 units/mg protein (E1%/280, 3'-5'-Phosphodiesterase)

Nuclease P₁ from Penicillium citrinum