N9527
Sigma

Monoclonal Anti-NTF2 antibody produced in mouse

~2 mg/mL, clone 4F5, purified immunoglobulin, buffered aqueous solution

Synonym

Anti-Nuclear Transfer Factor 2

MDL number

MFCD01633927

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Descriptions

Analysis Note

The antibody may also be used for microinjection, in vitro nuclear protein import assays, and blocking of NTF2 binding to Ran.

Immunogen

recombinant human NTF2. The epitope lies within the extreme C-terminus of NTF2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

4F5

form

buffered aqueous solution

mol wt

antigen mol wt 14 kDa

species reactivity

human, monkey, hamster, rat, mouse, chicken, Xenopus

concentration

~2 mg/mL

application(s)

immunoblotting: 1-2 μg/mL using HeLa cells cytosol preparation

immunocytochemistry1: suitable using 3.7% formaldehyde-0.2% Triton X-100 or 4% paraformaldehyde-methanol.

immunoprecipitation: suitable

indirect ELISA: suitable

microarray: suitable

isotype

IgG2b

Gene Information

mouse ... Ntf2(104255)

rat ... NTF2(291981)

human ... NUTF2(10204)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Steggerda, S.M., et al. Mol. Biol. Cell 11, 703, (2000) abstract

Safety

WGK Germany

NWG

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Related Categories

... Antibodies for Gene Regulation and Expression > Antibodies to Nuclear Proteins

... Alphabetical Index > N

... Clone Index > 4

N9527 - Datasheet (21kb)

MSDS

Certificate of Analysis

More Information