Features and Benefits
• From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
• Up to 350 μg (Midi) and 1.2 mg (Maxi) yield of high-copy plasmid DNA
• Offers the flexibility of a vacuum or spin format
• Contains fewer plastic components than other high speed kits, reducing the amount of waste
• No phenol/chloroform extraction or alcohol precipitation required
Other Notes
Principle
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. The lysate is cleared with a filter syringe and DNA is bound to the silica-based membrane. The remaining contaminants are removed by a wash step. Finally, the bound DNA is eluted in buffer or water. This high quality DNA is suitable for the most demanding applications including transfection, restriction digestion, ligation, automated sequencing.
Legal Information
GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
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