Features and Benefits
• Recoveries up to 80%
• Each column can bind up to 10 μg of DNA
• Up to 3.5 g can be processed per column
• Compatible with both standard and low-melting agarose in TAE or TBE buffer
Other Notes
The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.
Principle
The GenElute Gel Extraction Kit combines silica- binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel by solubilizing the gel. The Gel Solubilization Solution can dissolve an agarose gel slice from gels run in either TBE or TAE buffer. Extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer. The isolated DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR?, restriction digestion, cloning, and labeling.
Legal Information
GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
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