Nitric Oxide Synthase Detection Kit, Isotopic 

Upon receipt, store Box 1 at −70 °C, Box 2 at room temperature.

Sigma's Nitric Oxide Synthase Detection System is a simple and specific assay for the measurement of nitric oxide synthase (NOS) activity. Nitric oxide (NO), formed from the amino acid L-arginine via the action of the NOS enzyme, is a unique cell signaling molecule that functions as both an intracellular and an extracellular messenger. NO has been implicated in vasodilation, neurotransmission, cytotoxicity, and inflammation.¹
This kit utilizes a highly specific and sensitive assay for the direct quantitative measurement of nitric oxide synthase activity. The assay measures the enzymatic conversion of ³H-arginine to ³H-citrulline and is the standard assay for measuring NOS activity in cell lysates, tissue homogenates and purified enzyme preparations. Activity is measured in the picomole range and a positive control is included.

sufficient for 50 assays

nuclease and protease, free

Stuehr, D.J., Structure-function aspects in the nitric oxide synthases. Annu. Rev. Pharmacol. Toxicol. 37, 339-359, (1997) abstract

Geller, D.A., and Billiar, T.R., Molecular biology of nitric oxide synthases. Cancer Metastasis Rev. 17, 7-23, (1998) abstract

Bredt, D.S., and Snyder, S.H., Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum. Proc. Natl. Acad. Sci. USA 86, 9030-9033, (1989) abstract

Bredt, D.S., Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme. Proc. Natl. Acad. Sci. USA 87, 685, (1990)

Marletta, M.A., et al., Catalysis by nitric oxide synthase. Curr. Opin. Chem. Biol. 2, 656-663, (1998) abstract

Spin columns and caps 50 each

Microcentrifuge tubes 50 each