Oligo(dT)-Cellulose 

For polyadenylated mRNA purification using rapid and simple chromatographic techniques. Covalently attached oligothymidylic acid chains are approx. 20 nucleotides long. Eukaryotic mRNA specifically binds by its 3′-poly(A) tail. Non-polyadenylated RNA and other contaminants can be removed by washing, and the purified mRNA is eluted with a low salt buffer.

RNase, none detected

−20°C

Aviv, H., Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Natl. Acad. Sci. USA 69, 1408, (1972) abstract

Krystosek, A., Improved methods for purification and assay of eukaryotic messenger ribonucleic acids and ribosomes. Quantitative analysis of their interaction in a fractionated reticulocyte cell-free system. J. Biol. Chem. 250, 6077, (1975) abstract

Gilham J. Am. Chem. Soc. 86, 4982, (1964)

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