O4264
Sigma

Osteopontin human

recombinant, expressed in mouse NSO cells, ≥95% (SDS-PAGE), lyophilized powder, cell culture tested

Synonym

Bone Sialoprotein-1

Early T Lymphocyte Activation Protein-1 (ETA-1)

OPN

Secreted Phosphoprotein 1 (Spp1)

MDL number

MFCD03100698

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Descriptions

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Biochem/physiol Actions

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity.

Properties

recombinant

expressed in mouse NSO cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

mol wt 60-65 kDa by SDS-PAGE (reducing)

total impurities

Endotoxin, tested

suitability