Calmodulin from bovine heart 

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

lyophilized powder

Protein, ~90% Lowry

cow ... CALM1(520277)

−20°C

Watterson, D.M., The complete amino acid sequence of the Ca²⁺-dependent modulator protein (calmodulin) of bovine brain. J. Biol. Chem. 255, 962, (1980)

Cheung, W.Y., Calmodulin plays a pivotal role in cellular regulation. Science 207, 19-27, (1980)

Klee, C.B., Isolation and characterization of bovine brain calcineurin: a calmodulin-stimulated protein phosphatase. Meth. Enzymol. 102, 227, (1983)

Stull, J.T., Ca²⁺/calmodulin-dependent myosin light-chain kinases. Adv. Second Messenger Phosphoprotein Res. 31, 141, (1997)

Lee, H.C., Sensitization of calcium-induced calcium release by cyclic ADP-ribose and calmodulin. J. Biol. Chem. 270, 9060, (1995)

Tanaka, Y., Tashjian, A.H., Jr., Calmodulin is a selective mediator of Ca²⁺-induced Ca²⁺ release via the ryanodine receptor-like Ca²⁺ channel. Proc. Natl. Acad. Sci. USA 92, 3244, (1995)

Nairn, A.C., and Picciotto, M.R., Calcium/calmodulin-dependent protein kinases. Semin. Cancer Biol. 5, 295, (1994)

Carafoli, E., Biogenesis: plasma membrane calcium ATPase: 15 years of work on the purified enzyme. FASEB J. 8, 993, (1994)

Bredt, D., and Snyder, S.H., Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme. Proc. Natl. Acad. Sci. USA 87, 682, (1990)

Beavo, J.A., Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol. Rev. 75, 725-748, (1995)

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