P0389
Sigma

Penicillinase from Bacillus cereus

lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)

Synonym

β-Lactamase I from Bacillus cereus

β-Lactamase II

Cephalosporinase

Penicillin amido-β-lactam hydrolase

CAS Number

9001-74-5

Enzyme Commission (EC) Number

3.5.2.6 ( BRENDA | IUBMB )

MDL number

MFCD00131819

EG/EC Number

232-628-8

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Descriptions

Analysis Note

Protein determined by biuret.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Lyophilized powder containing phosphate and citrate buffer salts

Properties

form

lyophilized powder

activity

10-30 units/mg protein (using cephaloridine.)

composition

Protein, ~10%

storage temp.

2-8°C

References

Reference

Levy, G.B. Nature 166, 740, (1950)

Pollock, M.R. and Torriani, A.M. Comp. rend. Acad. Sci. (Paris) 237, 276, (1953)

Merck

Merck 13,7164

Safety

Hazard Codes

Risk Statements

42/43

Safety Statements

22-36/37

WGK Germany

10-21