P0515
Sigma

Phospholipase D from Arachis hypogaea (peanut)

Type II, lyophilized powder, ≥60 units/mg protein

Synonym

Lecithinase D

Phosphatidylcholine phosphatidohydrolase

CAS Number

9001-87-0

Enzyme Commission (EC) Number

3.1.4.4 ( BRENDA | IUBMB )

MDL number

MFCD00131897

EG/EC Number

232-639-8

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Descriptions

Analysis Note

Protein determined using biuret, unless otherwise indicated.

Biochem/physiol Actions

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Unit Definition

One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.

Physical form

Partially purified, lyophilized powder containing buffer salts

Properties

type

Type II

form

lyophilized powder

composition

Protein, ~30%

storage temp.

−20°C

References

Reference

Dennis, E.A., Boyer, P.D., ed., Phospholipases. The Enzymes New York , (1983) 6, 307

Yang. S.F., Phospholipase D from savoy cabbage. Meth. Enzymol. 14, 208, (1969)

Imamura, S., Horiuti, Y., Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose. J. Biochem. Tokyo 85, 79, (1979)

Safety

Safety Statements

WGK Germany