P0968
Sigma

Monoclonal Anti-p16^INK4a/CDKN2 antibody produced in mouse

clone DCS-50, ascites fluid

MDL number

MFCD01092440

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Descriptions

Immunogen

recombinant human p16 protein.¹

Specificity

The antibody reacts specifically with the C-terminal (last 12 amino acids) of human^1,^2,^3,⁴ p16 molecule (also known as p16^INK4, p16^INK4a, p16^MTS1, inhibitor of CDK4). An additional application for this antibody is microinjection into cells.¹

Properties

antibody form

ascites fluid

clone

DCS-50

mol wt

antigen mol wt 16 kDa

contains

15 mM sodium azide

species reactivity

human

application(s)

immunoblotting: 1:1,000 using a cultured human tumor cell line extract

immunocytochemistry: suitable

immunohistochemistry (frozen sections): suitable

immunoprecipitation: suitable

indirect ELISA: suitable

microarray: suitable

isotype

IgG1

Gene Information

human ... CDKN2A(1029)

shipped in

dry ice

storage temp.

−20°C

References

Cited Reference

1. Lukas, J., et al., Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16. Nature 375, 503-506, (1995) abstract

2. Aagaard, L., et al., Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines. Int. J. Cancer 61, 115-120, (1995) abstract

3. Reed, A.L., et al., High frequency of p16^{(CDKN2/MTS-1/INK4A)} inactivation in head and neck squamous cell carcinoma. Cancer Res. 56, 3630-3633, (1996) abstract

4. Lukas, J., et al., Oncogenic aberrations of p16^INK4/CDKN2 and cyclin D1 cooperate to deregulate G1 control. Cancer Res. 55, 4818-4823, (1995) abstract

Safety

WGK Germany

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... Antibodies for Cell Cycle > Antibodies to Cell Cycle Regulators

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... Serine/Threonine Kinase Antibodies > Cyclin-Dependent Kinase Inhibitor (CKI)

... Clone Index > D