P1432
Sigma

Peroxidase from Glycine max (soybean)

essentially salt-free, lyophilized powder, 50-150 units/mg solid

Synonym

Donor: hydrogen-peroxide oxidoreductase

CAS Number

9003-99-0

Enzyme Commission (EC) Number

1.11.1.7 ( BRENDA | IUBMB )

MDL number

MFCD00071339

EG/EC Number

232-668-6

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Descriptions

Other Notes

Using 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) tablets (Prod. No. A 9941) as substrate, approx. four times the activity is observed.

Unit Definition

One unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C,. This purpurogallin (20 sec) unit is equivalent to ~18 μM units per min at 25 °C.

Properties

form

essentially salt-free, lyophilized powder

absorbance ratio

RZ ≥0.5

storage temp.

2-8°C

References

Reference

Hewson, W.D. and Hager, L.P. The Porphyrins 7, 295, (1979)

Shinmen, Y., Asami, J., Amachi, T., Shimizu, S., and Yamada, H. Agric. Biol. Chem. 50, 247, (1986)

Paul, K.G. The Enzymes 2nd ed.,, (1963) 8, 227

Everse, J., Everse, K.E., and Grisham, M.B., ed. Peroxidase Chem. Biol. Boca Raton, FL , (1991) 2

Olsen, R.L. and Little, C. Biochem. J. 222, 701, (1984)

Safety

WGK Germany