Placenta Growth Factor human 

The biological activity is measured by the ability to bind rhFlt-1/Fc in a functional ELISA. Immobilized rhFlt-1/Fc at 2 μg/mL (100 μL/well) can bind rhP/GF with a linear range at 0.1 - 5 ng/mL.

PlGF is expressed in the placenta and somewhat less in the heart, lung and thyroid gland. Placentally expressed PlGF may act as an autocrine on trophoblasts, which express both PlGF and its receptor (VEGFR-1). Since these cells also make VEGF-A, natural heterodimers (PlGF/VEGF-A) have also been detected. Two alternatively spliced isoforms of PlGF have been identified.

Reconstitute with sterile PBS containing at least 0.1% HSA or BSA. The rhP/GF concentration should be no less than 10 μg/mL.

Lyophilized from 81.7 μL of a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid (pH 7.4) containing 50 μg bovine serum albumin per 1 μg of cytokine.

expressed in Escherichia coli

≥97% (SDS-PAGE)

powder

mol wt 29 kDa

Endotoxin, tested

cell culture tested

−20°C

Conn, G., et al., Purification of a glycoprotein vascular endothelial cell mitogen from a rat glioma-derived cell line. Proc. Natl. Acad. Sci. USA 87, 1323-1327, (1990) abstract

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