Monoclonal Anti-Polyglutamines antibody produced in mouse 

GST-human Huntingtin (N-terminal fragment of 171 amino acids containing 65Q).

The antibody recognizes homomeric polyglutamines independent of the species.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

This product is for in vitro use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California. This product is sold under license to U.S. Patent No. 6,29,652, owned by the Regents of the University of California.

purified immunoglobulin

3B5H10

buffered aqueous solution

human

~2 mg/mL

immunoblotting: 1-2 μg/mL using extract of HEK-293T cells transfected with an N-terminal 171 amino acid fragment of human Huntingtin with a 68 glutamine stretch

immunocytochemistry: suitable

immunoprecipitation: suitable

IgG1

human ... HD(3064)

dry ice

−20°C

Brooks, E., et al., Using antibodies to analyze polyglutamine stretches. Methods Mol. Biol. 277, 103-128, (2004) abstract

Wacker, J.L., et al., Hsp70 and Hsp40 attenuate formation of spherical and annular polyglutamine oligomers by partitioning monomer. Nat. Struct. Biol. 11, 1215-1222, (2004)

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