Peroxidase Anti-Peroxidase Soluble Complex antibody produced in goat 

Solution in 8 mM sodium acetate, 15 mM ammonium acetate buffered saline, pH 7.2, containing 0.5% bovine serum albumin and 15 mM sodium azide.

Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.

The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.

affinity isolated antibody

buffered aqueous solution

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

dry ice

−20°C

Sternberger, L.A. J. Histochem. Cytochem. 18, 315, (1970) abstract

Sternberger, L.A. Immunochemistry New York , (1979), 122

3