Protein C from human plasma 

Lyophilized powder from 20 mM Tris-HCl, pH 7.4, containing 0.1 M NaCl

Protein C is a plasma, vitamin K-dependent zymogen of a serine protease that can inhibit blood coagulation by inhibiting thrombin formation, selectively inactivating Factors Va and VIIIa. The Protein C anticoagulant pathway is triggered when thrombin binds to the endothelial cell proteoglycan, thrombomodulin. This complex, which cannot clot blood, is a potent activator of the protein C zymogen. Activation involves the release of a dodecapeptide from the N-terminal domain of the heavy chain. The activated Protein C (APC) then binds to protein S on cell surfaces and inactivates the coagulation factors Va and VIIIa by proteolysis. APC has also been shown to bind to receptors on the endothelium of large blood vessels.

≥90% (SDS-PAGE)

lyophilized powder

heavy chain mol wt 41 kDa

light chain mol wt 21 kDa

H₂O: 1 mg/mL

human ... PROC(5624)

−20°C

Walker, F.J., and Fay, P.J., Regulation of blood coagulation by the protein C system. FASEB J. 6, 2561-2567, (1992)

Thorelli, E., et al., Cleavage of factor V at arg 506 by activated protein C and the expression of anticoagulant activity of factor V. Blood 93, 2552-2558, (1999)

Kisiel, W., Human plasma protein C: isolation, characterization, and mechanism of activation by alpha-thrombin. J. Clin. Invest. 64, 761-769, (1979)

Esmon, C.T., and Fukudome, K., Cellular regulation of the protein C pathway. Semin. Cell. Biol. 6, 259-268, (1995)

Heeb, M.J., et al., Importance of individual activated protein C cleavage site regions in coagulation factor V for factor Va inactivation and for factor Xa activation. Eur. J. Biochem. 260, 64-75, (1999)

Laszik, Z., et al., Human protein C receptor is present primarily on endothelium of large blood vessels: implications for the control of the protein C pathway. Circulation 96, 3633-3640, (1997)

Esmon, C.T., et al., Regulation and functions of the protein C anticoagulant pathway. Haematologica 84, 363-368, (1999)

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