P2438
Sigma

Immobilon^™-P Polyvinylidene Difluoride membranes

size 9 cm × 12 cm

Application

Polyvinylidene difluoride membranes are popular membranes for immunoblotting (Western blotting),¹ and sequencing² of proteins. The basis of protein binding to PVDF is believed to be due to hydrophobic interactions. PVDF membranes for Western blotting are available in the most commonly used 0.45 μm pore size. PVDF membranes have a greater protein binding capacity, are more durable and more solvent resistant than nitrocellulose.

Legal Information

Immobilon is a trademark of Millipore Corp.

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Identifiers

Synonym

PVDF

Properties

size

9 cm × 12 cm

References

Cited Reference

1. Pluskal, M.G., et al., Immobilon PVDF transfer membrane: A new membrane substrate for Western blotting of proteins. Biotechniques 4, 272-282, (1986)

2. Hirano, H., et al., Microsequence analysis of the N-terminally blocked proteins immobilized on polyvinylidene difuoride membrane by Western blotting. Electrophoresis 14, 839-846, (1993)

Reference

Suck, R.W.L. and Krupinska, K., Repeated probing of Western blots obtained from Coomassie Brilliant blue-stained or unstained polyacrylamide gels. Biotechniques 21, 418-422, (1996)

Safety

WGK Germany

Related Categories

... Electrophoresis Equipment > Blotting Membranes and Paper

... Millipore > Electrophoresis

... Membranes & Blotting Paper > Immobilon™ PVDF Membranes

Proteomics Prod Guide (138kb)

MSDS

Certificate of Analysis

More Information

Immobilon™-P Polyvinylidene Difluoride membranes

size 9 cm × 12 cm

Immobilon^™-P Polyvinylidene Difluoride membranes