P2859
Sigma

Monoclonal Anti-p300/CBP antibody produced in mouse

~2 mg/mL, clone NM11, purified immunoglobulin, buffered aqueous solution

Synonym

Anti-CREB Binding Protein

MDL number

MFCD02097119

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Descriptions

Immunogen

native human p300.

Specificity

Reacts specifically with both p300 and CBP but not with the related p270 molecule. The epitope recognized by the antibody resides within the 21 amino acid stretch spanning amino acids 2071-2091 near the CBP C-terminus. CBP and p300 differ by three noncontiguous residues within this 21 amino acid region, a difference that does not detectably affect the reactivity of the antibody.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

NM11

form

buffered aqueous solution

mol wt

antigen mol wt 300 kDa

species reactivity

human, monkey, mink, mouse, rat

concentration

~2 mg/mL

application(s)

immunoblotting1,2: 10-20 μg/mL using human 293 embryonal kidney cells

immunocytochemistry: suitable

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

immunohistochemistry (frozen sections): suitable

immunoprecipitation1,2: suitable

microarray: suitable

isotype

IgG1

Gene Information

mouse ... Pcaf(18519)

rat ... Pcaf(301164)

human ... PCAF(8850)

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany

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P2859 - Datasheet (34kb)

MSDS

Certificate of Analysis

More Information