Features and Benefits
• Reduce set-up time, eliminate contamination concerns, and avoid long reactivation steps with JumpStart Readymixes
• JumpStart Taq antibody allows for room temperature set up of reactions making it ideal for high throughput applications.
• Prevents amplification of nonspecific products and primer dimers, increasing target yields.
Other Notes
JumpStart™ Taq ReadyMix™ is a prepared solution combining the performance benefits of hot start PCR with the convenience of a ReadyMix. The mix includes JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. JumpStart Taq Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq polymerase does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction. The hot start mechanism allows for room temperature set up, making it ideal for high throughput applications. To prepare a 50 μL reaction, simply add 25 μL of ReadyMix to template, primers and water for a final reaction volume of 50 μL.
Legal Information
JumpStart is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
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