Application
A convenient, ready to use, platform for the capture and detection of FLAG® fusion proteins. The ANTI-FLAG® M2 antibody is covalently attached to the surface through the Fc portion and can detect 1 ng FLAG fusion protein/well with a capacity of up to 300 ng/well. Suitable for screening for expression, study of protein:protein interactions and ELISA assays. Manufactured under ISO 9002 in Sigma's GMP facility, ANTI-FLAG® high sensitivity M2 coated multiwell plates utilize a flat bottom, polystyrene baseplate.
Other Notes
Specificity
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent.
Legal Information
ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.