P3125
Sigma

Papain from papaya latex

buffered aqueous suspension, 2× Crystallized, 16-40 units/mg protein

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Synonym

Papainase

CAS Number

9001-73-4

Enzyme Commission (EC) Number

3.4.22.2 ( BRENDA | IUBMB )

MDL number

MFCD00131791

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Descriptions

Application

Used to produce Fab fragments of antibodies.¹ Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.^2,³

Biochem/physiol Actions

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Physical form

Suspension in 0.05 M sodium acetate, pH 4.5, containing 0.01% thymol

Properties

form

buffered aqueous suspension

quality

2× Crystallized

mol wt

mol wt 21 kDa

storage temp.

2-8°C

References

Cited Reference

1. Y. Ozari, J. Jagur-Grodzinski J. Chem. Soc., Chem. Commun., 295, (1974)

2. M.A. Andrews Carbohydr. Res. 194, 1, (1989) abstract

3. H. Cai et al. Anal. Chem. 70, 580, (1998)

Reference

Townes-Anderson, E., et al., Rod Cells Dissociated from Mature Salamander Retina: Ultrastructure and Uptake of Horseradish Peroxidase. J. Cell Biol. 100, 175, (1985)

Dreyfus, Cheryl F., Black, Ira B., and, P. Michael Conn, ed. Methods in Neuroscience San Diego , (1990) 2, 10

, Harlow, E., and Lane, D. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1988), 626-628

Safety

Hazard Codes

Risk Statements

42/43

Safety Statements

WGK Germany