P4039
Sigma

Phospholipase C from Clostridium perfringens (C. welchii)

Type XIV, lyophilized powder, ≥150 units/mg protein

Synonym

Lecithinase C

Lipophosphodiesterase I

PC-PLC

Phosphatidylcholine cholinephosphohydrolase

CAS Number

9001-86-9

Enzyme Commission (EC) Number

3.1.4.3 ( BRENDA | IUBMB )

MDL number

MFCD00131895

EG/EC Number

232-638-2

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Descriptions

Biochem/physiol Actions

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Preparation Note

Chromatographically purified

Unit Definition

One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

Physical form

Lyophilized powder in buffered salts

Properties

type

Type XIV

form

lyophilized powder

Gene Information

Clostridium perfringens str. 13 ... plc(988262)

storage temp.

−20°C

References

Reference

Dennis, E.A., Boyer, P.D., ed., Phospholipases. The Enzymes New York , (1983) 16, 307

Takahashi, T., et al., Phospholipase C from Clostridium perfringens. Meth. Enzymol. 71, 710-725, (1981)

Zwaal, R.F., Roelofsen, B., Phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) from Bacillus cereus. Meth. Enzymol. 32, 154, (1974)

Safety

Safety Statements

22-24/25

WGK Germany

RTECS

SZ6116000

3-10