ReadyMix^™ Taq PCR Reaction Mix 

• Amplifies targets up to 7 kb in length
• ReadyMix Taq PCR Reaction Mix is provided either with or without MgCl₂ in the mixture. A separate vial of 25 mM MgCl₂ is provided for optimization with the ReadyMix Taq PCR Reaction Mix without MgCl₂
• ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient design
• REDTaq^® ReadyMix PCR Reaction Mix combines all the advantages of the ReadyMix with the added convenience of a direct load system. After PCR, an aliquot can be removed from the reaction and loaded directly onto an agarose gel with no loading dye additions, making it ideal for high throughput applications

ReadyMix^™ Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma's high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μl) to the primers, template and water (total volume 50 μl). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma's Taq DNA Polymerase.

Supplied with a vial of PCR grade water for dilution.

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

REDTaq is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

1.5 units/reaction (50 μl reaction volume)

wet ice

−20°C

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