P4912
Sigma

Phospholipase D from Streptomyces sp.

Type VII, lyophilized powder, ≥150 units/mg solid

Synonym

Lecithinase D

Phosphatidylcholine phosphatidohydrolase

CAS Number

9001-87-0

Enzyme Commission (EC) Number

3.1.4.4 ( BRENDA | IUBMB )

MDL number

MFCD00131897

EG/EC Number

232-639-8

Expand/Collapse All

Descriptions

Analysis Note

Protein by biuret

Biochem/physiol Actions

Glycerophospholipid-specific; markedly less active on sphingomyelins and lysophospholipids.

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Unit Definition

One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.

Properties

type

Type VII

form

lyophilized powder

storage temp.

−20°C

References

Reference

Dennis, E.A., Boyer, P.D., ed., Phospholipases. The Enzymes New York , (1983) 6, 307

Imamura, S., Horiuti, Y., Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose. J. Biochem. Tokyo 85, 79, (1979)

Yang. S.F., Phospholipase D from savoy cabbage. Meth. Enzymol. 14, 208, (1969)

Safety

Safety Statements

WGK Germany