Pleiotrophin human 

The activity is measured in culture by the ability to enhance neurite outgrowth of cerebral cortical neurons from chick embryos.

Pleiotrophin may be the first member of a family of developmentally regulated cytokines. It is active in growth and development and the pleiotrophin gene is highly expressed in brain, uterus, gut, muscle, lung, and skin. Pleiotrophin mRNA is expressed in osteoblasts, chondrocytes, fibroblasts, astrocytes, Schwann cells, and tumor cells. Pleiotrophin is mitogenic and displays neurite outgrowth activity. It stimulates tumor angiogenesis. Pleiotrophin is also known as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding growth-associated molecule (HB-GAM) and osteoblast-specific factor (OSF-1). There is extraordinary conservation between the amino acid sequences of bovine, human, and rat pleiotrophin.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

expressed in Sf21 cells

≥97% (SDS-PAGE)

lyophilized powder

predicted mol wt 15.3 kDa

Endotoxin, tested

cell culture tested

human ... PTN(5764)

−20°C

Merenmies, J., et al., Molecular cloning of the 18-kDa growth-associated protein of developing brain. J. Biol. Chem. 265, 16721-16724, (1990) abstract

Hampton, B.S., Structural and functional characterization of full-length heparin-binding growth associated molecule. Biol. Cell 3, 85, (1992)

Deuel, T.F., et al., Pleiotrophin: a cytokine with diverse functions and a novel signaling pathway. Arch. Biochem. Biophys. 397, 162-171, (2002) abstract

Nobuaki, M., and Masaharu, N., Involvement of receptor-like protein tryosine phosphatase zeta/RPTPbeta and its ligand pleiotrophin/heparin-binding growth-associated molecule (HB-GAM) in neuronal migration. J. Cell Biol. 142, 203, (1998) abstract

Zhang, N., et al., Domain structure of pleiotrophin required for transformation. J. Biol. Chem. 274, 12959, (1999) abstract

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