P5542
Sigma

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

Synonym

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming

PI-PLC

CAS Number

37288-19-0

Enzyme Commission (EC) Number

4.6.1.13 ( BRENDA | IUBMB )

MDL number

MFCD00131896

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Descriptions

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

Properties

form

lyophilized powder

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein

Sphingomyelinase ≤40 units/mg protein

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

storage temp.

−20°C

References

Reference

Takos, A.M., et al., Glycosyl-phosphatidylinositol-anchor addition signals are processed in Nicotiana tabacum. Plant J. 21, 43, (2000)

Coonrod, S.A., Treatment of mouse oocytes with PI-PLC releases 70-kDa (pI 5) and 35- to 45-kDa (pI 5.5) protein clusters from the egg surface and inhibits sperm-oolemma binding and fusion. Dev. Biol. 207, 334, (1999)

Sundler, R., et al., Enzymatic properties of phosphatidylinositol inositolphosphohydrolase from Bacillus cereus. Substrate dilution in detergent-phospholipid micelles and bilayer vesicles. J. Biol. Chem. 253, 4175, (1978)

Ellman, G.L., et al. Biochem. Pharmacol. 7, 88, (1961)

Vai, M., et al., The cell cycle modulated glycoprotein GP115 is one of the major yeast proteins containing glycosylphosphatidylinositol. Biochim. Biophys. Acta 1038, 277-285, (1990)

Safety

Safety Statements

22-24/25

WGK Germany