HIS-Select^® Nickel Affinity Gel 

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Nickel Affinity Gel used for the purification of histidine-tagged proteins. The unique, non-charged, hydrophilic linkage of the NTA chelate group to the agarose ensures high selectivity for histidine-tagged proteins and reduced non-specific binding of other proteins. Its high selectivity allows for one-step purification. Affinity gel can be used in either denaturing or non-denaturing conditions.

• High selectivity for higher purity
• Unique non-charged hydrophilic linkage reduced non-specific binding
• Binding capacity for histidine-tagged protein is greater than 15 mg/ml
• Binding under denaturing or non-denaturing conditions
• One-step purification

It is also available with the EZview™ technology (Product Code E3528).

50% suspension in 30% ethanol

Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.

HIS-Select is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Sepharose is a registered trademark of GE Healthcare.

6% Beaded Agarose

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

2-8°C

UN 1170 3/PG 3

1

89 °F

32 °C