Monoclonal Anti-CD4−PE antibody produced in mouse 

CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56^lck protein tyrosine kinase.

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.

Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

purified immunoglobulin

Q4120

buffered aqueous solution

antigen mol wt 59 kDa

human

flow cytometry: 10 μL using 1 × 10⁶ cells

IgG1

human ... CD4(920)

wet ice

2-8°C

Sattentau, Q.J., and Weiss, R.A., The CD4 antigen: physiological ligand and HIV receptor. Cell 52, 631, (1988)

Schlossman, S.F. Leucocyte Typing V Oxford , (1995)

Healey, D., et al., Novel anti-CD4 monoclonal antibodies separate human immunodeficiency virus infection and fusion of CD4+ cells from virus binding. J. Exp. Med. 172, 1233, (1990)

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