Phosphatase, Alkaline from bovine intestinal mucosa 

Package sizes are based on DEA units

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5'-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

lyophilized powder

homodimer mol wt ~160 kDa

−20°C

Reid, T.W., et al. Enzymes 3, 373-416, (1971)

Takanami, M., Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids 1. the 5'-termini of Escherichia coli ribosomal RNA. J. Mol. Biol. 23, 135, (1967)

22-24/25

3

N2770, SIGMAFAST^™ p-Nitrophenyl phosphate Tablets

M3168, 4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System

N1891, SIGMAFAST^™ p-Nitrophenyl phosphate Tablets

D6518, Diethylenetriaminepentaacetic acid

E4378, Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

P9375, 1,10-Phenanthroline monohydrate

ED2SS, Ethylenediaminetetraacetic acid disodium salt dihydrate