Monoclonal Anti-CD45−PE antibody produced in mouse 

CD45 leukocyte common antigen (LCA) family, which comprises at least four isoforms of membrane glycoproteins (220, 205, 190, 180 kDa) present on cells of the leukocyte lineage. These molecules, the intracellular portion of which have protein phosphatase activity, are involved in the regulation of transmembrane signals and enzymatic activation. CD45 is largely restricted to leukocytes.

non-T, non-B CALLA positive, and ALL cell line REH.

Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.

Recognizes the CD45 leukocyte common antigen (LCA) family. The CD45 antibody is therefore useful in distinguishing lymphomas from carcinomas. The epitope recognized by the antibody is sensitive to formalin fixation and paraffin embedding.
4th Workshop: code no. N817

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

purified immunoglobulin

BRA-55

buffered aqueous solution

human

flow cytometry: 10 μL using 1 × 10⁶ cells

IgG1

human ... PTPRC(5788)

wet ice

2-8°C

Chorvath, B., et al. Neoplasia 34, 685, (1987)

Knapp, W., et al. Leucocyte Typing IV Oxford , (1989)

Chorvath, B., et al. Neoplasia 35, 495, (1988)

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