Pyruvate Kinase from rabbit muscle 

Protein determined by biuret.

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.

Solution in 50% glycerol containing 0.01 M phosphate, pH 7.0

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path
Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

The Enhanced Quality Profile (EQP) is an SAFC program characterizing a product’s quality traits in a 5-level system to meet the requirements of our manufacturing customers. This product is in the PREMIUM level.

Type VII

buffered aqueous glycerol solution

lactic dehydrogenase, creatine phosphokinase, phosphoglucomutase, and myokinase <0.01%

2-8°C

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