Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit 

Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).

Package size based on protein content.

One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.0 at 30 °C.

Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl₂, Tween 20, and trehalose as stabilizer.

PP1 is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn²⁺ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).

expressed in Escherichia coli

lyophilized powder

mol wt 37.5 kDa

−20°C

Faux, M.C., and Scott, J.D., More on target with protein phosphorylation: conferring specificity by location. Trends Biochem. Sci. 21, 312-315, (1996) abstract

Zhang, Z., et al., Expression of the catalytic subunit of phosphorylase phosphatase (protein phosphatase-1) in Escherichia coli. J. Biol. Chem. 267, 1484-1490, (1992) abstract

Hunter, T., Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell 80, 225-236, (1995) abstract

Cohen, P., The structure and regulation of protein phosphatases. Annu. Rev. Biochem. 58, 453-508, (1989) abstract

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P8218, Protein Phosphatase Inhibitor-2 from rabbit

O7760, Okadaic acid sodium salt from Prorocentrum concavum

O7885, Okadaic acid potassium salt from Prorocentrum concavum

C5552, Calyculin A from Discodermia calyx

M2912, Microcystin LR from Microcystis aeruginosa

C1907, Calcineurin from bovine brain