P7962
Sigma

Monoclonal Anti-PSTAIR antibody produced in mouse

clone PSTAIR, ascites fluid

MDL number

MFCD00677382

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Descriptions

Immunogen

synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA.

Specificity

Recognizes an epitope present in the PSTAIR sequence of p34^cdc2(Cdk1), and of other cyclin-dependent kinases (Cdk2, Cdk3). Does not precipitate p34^cdc2 complexed with cyclin B.

Properties

antibody form

ascites fluid

clone

PSTAIR

mol wt

antigen mol wt 33-34 kDa

contains

15 mM sodium azide

species reactivity

lily, onion, human, monkey, mouse, Japanese quail, goldfish, carp, eel, starfish, amago salmon, Xenopus, ciliates

application(s)

immunoblotting: 1:4,000 using a COS-7 cell extract

immunoprecipitation: suitable

indirect ELISA: suitable

isotype

IgG1

Gene Information

human ... CDC2(983)

mouse ... Cdc2a(12534)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Yamashita, M., et al. Dev. Growth Differ. 33, 617, (1991)

Yamashita, M., et al. Dev. Growth Differ. 33, 625, (1991)

Hirai, T., et al., Isolation and characterization of goldfish cdk2, a cognate variant of the cell cycle regulator cdc2. Dev. Biol. 152, 113-120, (1992) abstract

Hirai, T., et al., Cyclin B in fish oocytes: its cDNA and amino acid sequences, appearance during maturation, and induction of p34^cdc2 activation. Mol. Reprod. Dev. 33, 131-140, (1992) abstract

Yasuda, H., et al., Cyclin-dependent kinase 2 (cdk2) in the murine Cdc2 kinase TS mutant. Som. Cell Mol. Gen. 18, 403-408, (1992)

Yamashita, M., et al., Purification and characterization of maturation-promoting factor in fish. Dev. Biol. 149, 8-15, (1992) abstract

Yamashita, M., et al., M-phase-specific histone H1 kinase in fish oocytes. Purification, components and biochemical properties. Eur. J. Biochem. 205, 537-543, (1992) abstract

Mori, M., et al., Maturation-promoting factor and p34cdc2 kinase during oocyte maturation of the Japanese quail. Dev. Biol. 146, 246-249, (1991) abstract

Safety

WGK Germany