P7965
Sigma

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse

clone F4, ascites fluid

MDL number

MFCD00282355

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Descriptions

Immunogen

mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Specificity

The antibody recognizes an epitope located in the amino terminal half of P-glycoprotein (Pgp), at the third extracellular loop of the molecule. The epitope is resistant to formalin fixation and periodate oxidation.¹ The antibody detects specifically human MDR1 P-glycoprotein, but does not appear to recognize the human MDR3 product¹, nor the mouse mdr1a, mdr1b or the mdr3 P-glycoprotein.²

Properties

antibody form

ascites fluid

clone

F4

mol wt

antigen mol wt 170-180 kDa

contains

15 mM sodium azide

species reactivity

human, hamster

application(s)

immunoblotting: suitable

immunocytochemistry: suitable

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections

immunohistochemistry (frozen sections): suitable

immunoprecipitation: suitable

indirect ELISA: suitable

radioimmunoassay: suitable using cell-surface RIA

isotype

IgG1

Gene Information

human ... ABCB1(5243)

shipped in

dry ice

storage temp.

−20°C

References

Cited Reference

1. Chu, T.M., et al. Hybridoma 12, 417, (1993) abstract

2. Chu, T.M., et al. Biochem. Biophys. Res. Commun. 203, 506, (1994) abstract

Safety

WGK Germany

3

Related Categories

... Antibodies for Multi-Drug Resistance > Antibodies to Drug Efflux Pumps

... Clone Index > F

... Alphabetical Index > G

P7965 - Datasheet (84kb)

MSDS

Certificate of Analysis

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