Platelet-Derived Growth Factor from human platelets 

The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.

The principal mitogen found in mammalian serum, released from platelets during clot formation. Elicits mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. Mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. Appears to interact with transforming growth factor-β1 in accelerating wound healing. However, PDGF may also be pathogenic in arteriosclerosis and neoplasia.

Isolated from human platelets, predominantly containing two covalently linked subunits (A and B).

Lyophilized from acetic acid solution

≥95% (SDS-PAGE)

lyophilized powder

0.5-10.0 ng/mL EC₅₀

glycoprotein mol wt 28-31 kDa

Endotoxin, tested

cell culture tested

−20°C

Ross, R., et al., A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc. Natl. Acad. Sci. USA 71, 1207-1210, (1974)

Raines, E., et al., Purification of human platelet-derived growth factor. Meth. Enzymol. 109, 749-773, (1985)

Ross, R., Atherosclerosis: a problem of the biology of arterial wall cells and their interactions with blood components. Arteriosclerosis 1, 293-311, (1981)

Pierce, G., et al., Platelet-derived growth factor and transforming growth factor-beta enhance tissue repair activities by unique mechanisms. J. Cell Biol. 109, 429-440, (1989)

B

3