Protein Phosphatase Inhibitor-2 from rabbit 

Protein phosphatase inhibitor-2 is a specific inhibitor of protein phosphatase 1 (PP1). It constitutes the regulatory subunit of the cytosolic form of PP1 known also as ATP-Mg dependent phosphatase or protein phosphatase 1l (PP1l). Activation of PP1l can be achieved by phosphorylation of I-2 on the Thr-72 by glycogen synthase kinase-3 (GSK-3). This activation is increased by casein kinase phosphorylation in a synergistic mechanism.

Package size based on protein content.

One unit will inhibit 1 unit of protein phosphatase 1 (PP1) catalytic subunit by 50%. One unit of PP1 will release 1.0 nmol phosphate per min at pH 7.5 at 30 °C from phosphorylase a.

Lyophilized powder containing Tris buffer, pH 7, EDTA and leupeptin

expressed in Escherichia coli

lyophilized powder

mol wt ~31 kDa

Protein, ~15% Bradford

−20°C

Cohen, P. Annu. Rev. Biochem. 58, 453, (1989) abstract

Cohen, P., et al. Meth. Enzymol. 159, 427, (1988) abstract

Park, I.-K., et al. J. Biol. Chem. 269, 944, (1994) abstract

Plyte, S., et al. Biochim. Biophys. Acta 1114, 147, (1992) abstract

DePaoli-Roach, A. J. Biol. Chem. 259, 12144, (1984)

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