Peroxidase from horseradish 

The RZ (Reinheitszahl) is the absorbance ratio A₄₀₃/A₂₇₅ determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

Preliminary work shows it to contain at least five isoenzymes.

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca²⁺ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

The Enhanced Quality Profile characterizes an individual product's quality traits in a multilevel system to assure that a given product meets the quality requirements of our customers.

Type II

essentially salt-free, lyophilized powder

RZ ≥1.8

Premium

2-8°C

3

A4382, 4-Aminoantipyrine

A0156, N-(4-Aminobutyl)-N-ethylisoluminol

A1661, N-(6-Aminohexyl)-N-ethylisoluminol

A1888, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

A3537, 5-Aminosalicylic acid

A4685, Luminol sodium salt

A5754, 3-Amino-9-ethylcarbazole

A8264, 4-Aminophthalhydrazide

A8511, Luminol

D5907, Dicarboxidine dihydrochloride

D9143, o-Dianisidine

G5502, Guaiacol

S2002, Sodium azide

Q0125, Quercetin dihydrate