Protein Kinase Cα from rat brain 

One unit will transfer 1nmol of phosphate from ATP to HistoneH1 (H 5505) per min at pH 7.4 at 30°C.

Solution in 50% glycerol with 85 mM potassium phosphate buffer, pH 7.5, 0.5 mM EDTA, 0.5 mM EGTA, 5 mM DTT, and 0.2% Tween 20

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP₂) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca²⁺ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca²⁺-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

≥90% (SDS-PAGE)

buffered aqueous glycerol solution

mol wt 82 kDa by SDS-PAGE

15-40 units/mL

rat ... Prkca(24680)

dry ice

−70°C

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Weinstein, I.B., et al., The role of protein kinase C in signal transduction, growth control and lipid metabolism. Adv. Exp. Med. Biol. 400A, 313-321, (1997)

Liu, W.S., and Heckman, C.A., The sevenfold way of PKC regulation. Cell. Signal. 10, 529-542, (1998)

Toker, A., Signaling through protein kinase C. Front. Biosci. 3, D1134-D1147, (1998)

Dong, L.Q., et al., Primary structure, tissue distribution, and expression of mouse phosphoinositide-dependent protein kinase-1. J. Biol. Chem. 274, 8117, (1999)

Kanashiro, C.A., and Khalil, R.A., Signal transduction by protein kinase C in mammalian cells. Clin. Exp. Pharmacol. Physiol. 25, 974-985, (1998)