Peroxidase from horseradish 

The RZ (Reinheitszahl) is the absorbance ratio A₄₀₃/A₂₇₅ determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Preliminary isoelectric focusing data indicates this is primarily isoenzyme C

A further purification of Peroxidase TypeVI (P8375).

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

Chromatographically purified

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca²⁺ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Type XII

essentially salt-free, lyophilized powder

RZ >3.0

2-8°C

Chavez, C. and Flurkey, W. J. Chromatogr. Sci. 298, 169, (1984)

Reisfeld, R., et al. Nature 195, 281, (1962) abstract

3

A5754, 3-Amino-9-ethylcarbazole

A0156, N-(4-Aminobutyl)-N-ethylisoluminol

A1888, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

A4382, 4-Aminoantipyrine

D9143, o-Dianisidine

A3537, 5-Aminosalicylic acid

D5907, Dicarboxidine dihydrochloride

G5502, Guaiacol

A4685, Luminol sodium salt

A8511, Luminol

A1661, N-(6-Aminohexyl)-N-ethylisoluminol

A8264, 4-Aminophthalhydrazide

S2002, Sodium azide