P8590
Sigma

Monoclonal Anti-CD31 (PECAM-1) antibody produced in mouse

2 mg/mL, clone WM-59, purified immunoglobulin, buffered aqueous solution

Synonym

Anti-PECAM-1

Monoclonal Anti-CD31

Descriptions

Antigen Background

Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.

Immunogen

human cell line RC-2A, originally derived from myeloid leukemia cells.

Specificity

Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.
5th Workshop: code No. P025

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4

Properties

antibody form

purified immunoglobulin

clone

WM-59

sterility

sterile; 0.2 μm filtered

form

buffered aqueous solution

mol wt

antigen mol wt 130-140 kDa

species reactivity

human

concentration

2 mg/mL

application(s)

indirect immunofluorescence: 1:100 using endothelial cells in human placenta frozen sections

isotype

IgG1

Gene Information

human ... PECAM1(5175)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Newman, P.J., et al. Science 247, 1219, (1990)

Schlossman, S.F., et al. Leucocyte Typing V Oxford , (1995), 1250

Safety

WGK Germany

Related Categories

... Antibodies to Cytoskeleton > Antibodies to Adhesion Molecules and Related Proteins

... Analytical Cytology > Antibodies to Human Leukocytes

... Antibodies for Immune Cell Signaling and Blood > Antibodies to Human Leukocytes

... Hematopoietic Stem Cell Characterization/Differentiation > Antibodies to Leukocytes

... Cardiac Stem Cell Tracking > Cardiogenesis Markers

... Alphabetical Index > CB to CN

... Clone Index > W

P8590 - Datasheet (26kb)

MSDS

Certificate of Analysis

More Information