P8615
Sigma

Phosphoinositide 3-kinase p110γ human

>95% (SDS-PAGE), recombinant, expressed in insect cells, buffered aqueous glycerol solution

Synonym

Phosphatidylinositol 3-kinase p110γ human

PI3K p110γ human

MDL number

MFCD03457993

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Descriptions

Biochem/physiol Actions

PI 3-kinases have been implicated in diverse cellular responses triggered by mammalian cell surface receptors. Inhibits NF-κB, AP-1, and STAT1α mediated gene expression induced by IFN-γ resulting in inhibition of COX-2 and NOS ll (iNOS) expression.

Unit Definition

1 unit will transfer 1 nmol of phosphate/min, using phosphatidylinositol as substrate.

Physical form

Solution in 25 mM HEPES, pH 7.4, 100 mM NaCl, 0.5 mM MgCl2, 50% glycerol.

Properties

recombinant

expressed in insect cells

assay

>95% (SDS-PAGE)

form

buffered aqueous glycerol solution

activity

~4.5 units/mg protein

Gene Information

human ... PIK3CG(5294)

shipped in

dry ice

storage temp.

−70°C

References

Reference

Leopoldt, D., et al., G_β/γstimulates phosphoinositide 3-kinase-γ by direct interaction with two domains of the catalytic p110 subunit. J. Biol. Chem. 273, 7024, (1998) abstract

Stoyavov, B., et al., Cloning and characterization of a G protein-activated human phosphoinositide-3-kinase. Science 269, 690, (1995) abstract

Safety

Safety Statements

23-24/25

WGK Germany