Phospholipase A₂ from Streptomyces violaceoruber 

Protein determined by biuret.

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.

Lyophilized powder containing mannitol and Tris buffer.

lyophilized powder

−20°C

Dennis, E.A., Boyer, P.D., ed., Phospholipases. The Enzymes New York , (1983) 16, 307

Cottrell, R.C., Phospholipase A₂ from bee venom. Meth. Enzymol. 71, 698, (1981)

Menashe, M., et al., Hydrolysis of dipalmitoylphosphatidylcholine small unilamellar vesicles by porcine pancreatic phospholipase A₂. J. Biol. Chem. 261, 5328, (1986)

Yoshida, H., et al., Phospholipase A of sea snake Laticauda semifasciata venom. Isolation and properties of novel forms lacking tryptophan. J. Biochem. Tokyo 85, 379, (1979)

Cao, Y.Z., et al., The purification and characterization of a phospholipase A in hamster heart cytosol for the hydrolysis of phosphatidylcholine. J. Biol. Chem. 262, 16927, (1987)

3

D6518, Diethylenetriaminepentaacetic acid

E4378, Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

ED2SS, Ethylenediaminetetraacetic acid disodium salt dihydrate

P9375, 1,10-Phenanthroline monohydrate

D6908, DL-erythro-Dihydrosphingosine

D8008, 7,7-Dimethyl-(5Z,8Z)-eicosadienoic acid